Journal: Journal of Alzheimer's Disease

Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid-β and Tau in Alzheimer's Disease

doi: 10.3233/jad-2010-1216

Figure Lengend Snippet: Fig. 1. LCM of Aβ-associated and non-associated neurons from AD hippocampal tissue section. Left panel, immunostaining using FITC-conjugated Aβ antibody. Middle panel, Nissl stain of neurons (dark areas). Right panel, laser capture of Nissl-stained neurons that overlap with Aβ immunostaining were microdissected and collected as Aβ-associated neurons. Nissl-stained neurons without Aβ immunostaining were collected as Aβ-free neurons.

Article Snippet: After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated antirabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Pseudocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA).

Techniques: Immunostaining, Staining

Journal: Journal of Alzheimer's Disease

Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid-β and Tau in Alzheimer's Disease

doi: 10.3233/jad-2010-1216

Figure Lengend Snippet: Fig. 4. Hippocampal cells coexpressing Aβ and TrkB are present in AD and control cases. Representative DIF of Aβ/TrkB showing high levels of Aβ (green) in AD (AD5) compared to control (C5); higher levels of TrkB (red) in control compared to AD; and similar numbers of Aβ/TrkB (yellow cells in merge) in controls compared to AD (x200). Arrows point to examples of coexpressing cells. The bottom two panels are the negative controls using nonspecific mouse or rabbit IgG as primary antibodies and the appropriate FITC-avidin or Texas Red DCS. No immunostaining was detected.

Article Snippet: After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated antirabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Pseudocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA).

Techniques: Control, Avidin-Biotin Assay, Immunostaining